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专利名称：Method and device for rapid detection of
microorganisms by changing the shape ofmicro-colonies
发明人：Sergey Gazenko申请号：US11393012申请日：20060330
公开号：US20070238139A1公开日：20071011
专利附图：
摘要：This invention describes a method of rapid detection of micro-colonies ofmicroorganisms by changing their shape from a regular semi-sphere to a long and thin
cylinder. Cells are trapped by filtration in long (diameter/length=1/10-1/100), cylindrical,parallel, micro-channels that are open from both sides, and attached to a filter from oneside. A micro-channel plate houses a multiplicity of micro-channels (diameter of eachchannel=1 -20 μm, and length 100-1000 μm). The micro-channel plate with cells trappedon the surface of the filter is attached to a nutrient media agar block. Cells producemicro-colonies of a long and thin shape according the shape of the micro-channel. Thegrowth of microorganisms in the micro-channels permits a change in the number of cellsto accomplish light absorbance. Fewer cells need a shorter time to reproduce. Thusdetection and counting of cells can be accomplished in a rapid fashion. The lightabsorbance can be enhanced by additional coloration of micro-colonies by cell dyes orartificial chromogenic or fluorogenic substrates.
申请人：Sergey Gazenko
地址：Cincinnati OH US
国籍：US
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