专利内容由知识产权出版社提供
专利名称:Purification method and apparatus发明人:Christopher Peter Jones申请号:US08/5718申请日:19960104公开号:US06136555A公开日:20001024
摘要:A method is provided for purifying from cells a target compound such as nucleicacid. The method comprises the following steps: 1) lysing a cell suspension to form a celllysate containing the target compound; 2) applying the cell lysate to a filter to removeunwanted cells and cell debris; 3) contacting the filtered lysate with a solid phase matrixunder conditions to bind the nucleic acid to the matrix; 4) separating the resultantfiltered lysate from the matrix; and 5) eluting the target compound from the matrix.Apparatus is also provided. Complex purification procedures such as centrifugation areavoided.
申请人:CAMBRIDGE MOLECULAR TECHNOLOGIES LIMITED
代理机构:Burns, Doane, Swecker & Mathis, LP.
更多信息请下载全文后查看