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Purification method and apparatus

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专利名称:Purification method and apparatus发明人:Christopher Peter Jones申请号:US08/5718申请日:19960104公开号:US06136555A公开日:20001024

摘要:A method is provided for purifying from cells a target compound such as nucleicacid. The method comprises the following steps: 1) lysing a cell suspension to form a celllysate containing the target compound; 2) applying the cell lysate to a filter to removeunwanted cells and cell debris; 3) contacting the filtered lysate with a solid phase matrixunder conditions to bind the nucleic acid to the matrix; 4) separating the resultantfiltered lysate from the matrix; and 5) eluting the target compound from the matrix.Apparatus is also provided. Complex purification procedures such as centrifugation areavoided.

申请人:CAMBRIDGE MOLECULAR TECHNOLOGIES LIMITED

代理机构:Burns, Doane, Swecker & Mathis, LP.

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